strate p-nitrophenyl phosphate to produce a yellow-colored product, p-nitrophenol and inorganic phosphate: The initial velocity (v o) of the reaction, in micromoles of product produced/minute, can be calculated from the increase in absorb-ance at 400 nm (A 400) measured at timed intervals during the
Visit ChemicalBook To find more P-NITROPHENYL PHOSPHATE MAGNESIUM SALT(32348-90-6) information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. You can also browse global suppliers,vendor,prices,Price,manufacturers of P-NITROPHENYL PHOSPHATE MAGNESIUM …
para-Nitrophenylphosphate (pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays. Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm.
p-Nitrophenyl carbonates are highly reactive compounds that are usually treated with alcohols or amines to give either a new carbonate or a carbamate-linked compound depending on the nucleophile. In one of our earlier reports, polycarbamate nucleic acids were synthesized from p -nitrophenyl carbonates with amines of nucleic acid derivatives [ 18 ].
4-Nitrophenyl chloroformate 96% Synonym: Chloroformic acid 4-nitrophenyl ester CAS Number 7693-46-1. Linear Formula ClCO 2 C 6 H 4 NO 2. Molecular Weight 201.56 . Beilstein/REAXYS Number 518127 . EC Number 231-706-9. MDL number MFCD00007321. PubChem Substance ID 24849861
A <stereo>beta</stereo>-<stereo>D</stereo>-galactopyranoside having a 4-nitrophenyl substituent at the anomeric position. ChEBI CHEBI:355715 A beta-D-galactopyranoside having a 4-nitrophenyl substituent at the anomeric position.
ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol.
4-Nitrophenyl-β-D-glucopyranoside. Molecular Weight: 301.3 High purity 4-Nitrophenyl-β-D-glucopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of β-glucosidase activity.
Read "p-Nitrophenyl--D-xylopyranoside, Acta Crystallographica Section B: Structural Crystallography and Crystal Chemistry" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Other names: Hydrazine, (p-nitrophenyl)-; (p-Nitrophenyl)hydrazine; (4-Nitrophenyl)hydrazine; p-Hydrazinonitrobenzene; p-Nitrophenylhydrazide Permanent link for this species. Use this link for bookmarking this species for future reference. Information on this page: Notes; Other data available: IR Spectrum; Mass spectrum (electron ionization .
4-Nitrophenyl acetate is used with iodoacetic acid for reductive cleavage of methionine-containing peptides. It is also used as a substrate that has been used in assays for esterase and lipase activity. Inorganic complexes have been evaluated for their methanolysis …
p-nitrophenyl phosphate by an alkaline phosphatase preparation and by 1 N HCl. It will be noted that there is good agreement between the two methods and that essentially all of the substrate is hydrolyzed by the phosphatase. The molar extinction coefficient of p-nitrophenol is 17,500
p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases. The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol, a chromogenic product .
P-nitrophenyl butyrate is a butyrate ester resulting from the formal condensation of the hydroxy group of 4-nitrophenol with the carboxy group of butyric acid.It is a butyrate ester and a C-nitro compound. It derives from a 4-nitrophenol.
The concentration of the formed p-nitrophenolate ion was measured spectrophotometrically at 400 nm (ε = 18 300 M-1cm-1) The reaction was carried out at 200 C for 15 s and was stopped by the addition of 0.8 ml of 0.1 M NaOH solution. The measurements of the p-nitrophenolate absorbance were performed at 400 nm using a double beam UV-VIS Cary 300.
Amines linked to p-nitrophenyl carbonate Wang resin possess similar chemical properties to methoxybenzyloxycarbonyl protected amines. The resin-bound carbamate is, therefore, cleaved with TFA or hydrogenolysis to afford the free amine .
Search results for p-nitrophenyl at Sigma-Aldrich
pnpp,disodium 4-nitrophenylphosphate,sodium 4-nitrophenyl phosphate,disodium 4-nitrophenyl phosphate,phosphoric acid, mono 4-nitrophenyl ester, disodium salt,disodium p-nitrophenyl phosphate,phosphoric acid, mono 4-nitrophenyl ester, sodium salt 1:2,p-nitrophenyl phosphate,pnpp liquid substrate system: PubChem CID: 77949: IUPAC Name
versus p-nitrophenol concentration was prepared using the purified p-nitrophenol described above. RESLJLTS Order of the Reactiofz The reaction at 76.9' C. was found to be first order with respect to P-nitrophenyl P H FIG. 2. Tcn~perature = iF.9" C. p-Sitrophenyl phosphate = 0.831X10-3 >If.
4-Nitrophenyl-α-D- glucopyranoside is a chromogenic substrate for alpha-D-glucosidase detecting glucansucrases and useful in studies of concanavalin A.
p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases. The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol, a chromogenic product with absorbance at 405 nm.
1. The hydrolysis of p-nitrophenyl phosphate has been studied in human red cells, ghosts and haemoglobin-free membranes to see whether hydrolysis was related to the functioning of the sodium pump. 3. p-Nitrophenyl phosphate was hydrolysed in cells almost entirely by …
p-Nitrophenyl carbonates are highly reactive compounds that are usually treated with alcohols or amines to give either a new carbonate or a carbamate-linked compound depending on the nucleophile. In one of our earlier reports, polycarbamate nucleic acids were synthesized from p-nitrophenyl carbonates with amines of nucleic acid derivatives .
PNPA - P-Nitrophenyl Acetate. Looking for abbreviations of PNPA? It is P-Nitrophenyl Acetate. P-Nitrophenyl Acetate listed as PNPA. P-Nitrophenyl Acetate - How is P-Nitrophenyl Acetate abbreviated? . P-Nitrophenyl Acetate; P-Nitrophenyl-Alpha-D-Galacto-Pyranoside; P-Nitrophenyl-Thymidine 5'-Monophosphate; P-Nitrophenyldiphenyl Phosphate; P .
p-Nitrophenyl-N-acetyl-α-D-glucosaminide is commonly used as a chromogenic substrate for N-acetyl-β-D-glucosaminidase, yielding a yellow solution upon cleavage. Soluble in water, methanol.
4-Nitrophenyl-α-D- glucopyranoside is a chromogenic substrate for alpha-D-glucosidase, yielding a yellow solution upon cleavage. 4-Nitrophenyl-α-D- glucopyranoside is also used for the detection of glucansucrases and for yeast alpha-D-glucosidase and for crystal structure studies of the complexes of concanavalin A. Product Specifications.
Oct 10, 2019· Compound 4-Nitrophenyl chloroformate with free spectra: 1 NMR, 3 FTIR, 1 Raman, and 1 UV-Vis.
The Hazard fields include special hazard alerts, air and water reactions, fire hazards, health hazards, a reactivity profile, and details about reactive groups assignments and potentially incompatible absorbents.The information in CAMEO Chemicals comes from a variety of data sources.
p-Nitrophenyl Phosphate sc-222120 Hazard Alert Code Key: EXTREME HIGH MODERATE LOW! Accidental ingestion of the material may be damaging to the health of the individual.! The substance and/or its metabolites may bind to hemoglobin inhibiting normal uptake of oxygen. This condition, known as "methemoglobinemia", is a form of oxygen starvation .